Rameez Patel, 31 of Polokwane, who is accused of murdering his mother, Mahejeen, has been denied leave to appeal his rejected bail application, Netwerk24 reported.
Patel's lawyers maintained that he should be released on bail to care for his three young sons.
He is on trial for the murder of his mother Mahejeen (51), who was shot in the head in her home in Nirvana, Polokwane, on September 19 2017.
The state maintains that Patel paid a hitman R15 000 to kill his mother because of an alleged dispute over finances.
Patel brought his appeal after the Polokwane Magistrate's Court denied his application to be released on bail in February.
READ: Murder-accused Limpopo businessman denied bail
Members of the ANC Women's League filled the court benches on Thursday to show that they did not want Patel to be released on bail.
Patel was out on bail of R250 000 when he was arrested for the alleged murder of his mother on September 29 2017.
He was granted the R250 000 bail after being charged with the murder of his wife Fatima (28).
Fatima was murdered in the couple's townhouse in Nirvana on April 10. She had been hit with a baseball bat, strangled and then shot in the head with a 9mm pistol.
Patel has denied any involvement in the murder of either his wife or his mother.
READ: Rameez Patel in 2 courts for mother and wife's murders
Meanwhile Ali Salman (22), who allegedly shot Rameez's younger brother Razeen (24) outside Polokwane two weeks ago, was released on bail of R5 000 in the Mankweng Magistrate's Court.
Salman and Razeen were on their way home to Polokwane after a night on the town when Razeen was shot along the R71 road.
The cause of the shooting is still a mystery. Razeen was to have testified against Rameez in Fatima's trial in the Limpopo High Court the following day.
READ: Rameez Patel's brother shot a day before scheduled testimony
After being discharged from hospital, police granted Razeen permission to go to his brother Rakeeb in London.
Salman is due to appear in court again on September 7.